FastQCFastQC Report
Thu 28 Apr 2016
ERR1041863.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041863.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13251741
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT206860.156100243734012No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT195500.14752778521705184No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT138610.10459757702780337No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA96600.030.0864411
GTATCAA143000.020.1300682
GTATTAG14600.015.205481
CGCTATA12600.014.2420632
TATACTG15750.014.2126985
TATCAAC205200.014.1905463
GTATAGA14000.014.1392861
ATCAACG206600.013.9600684
TCAACGC206750.013.9409915
CAACGCA208850.013.8008146
CTACACT20550.013.5936744
TAATACG3850.013.4545454
AACGCAG216950.013.379357
CCTATAC12800.013.2968753
GTGTTAG23550.013.11889651
TACACTG25350.013.0631165
TTATACG4250.013.05882335
TCTATAC10450.012.9234453
CTATACT16100.012.8695654
TCGCTAT15500.012.6516121
Produced by FastQC (version 0.11.5)