FastQCFastQC Report
Thu 28 Apr 2016
ERR1041862.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041862.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2762373
Sequences flagged as poor quality0
Sequence length43
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTCAGAGAGGCGAGGGTTCGAGGGCACGAGTTCGAGGCCAA30280.10961589908386737No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT29610.1071904482124608No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT28510.10320836469224107No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18650.029.3619291
TATACGA753.744699E-722.236
GTATCAA27000.020.2129632
TATACGC555.1456055E-420.1818185
GTATACG656.906614E-519.9230771
TATAAGG1451.546141E-1017.8620682
TCGCTAA852.7253558E-517.41176414
CGTTAGA752.0685903E-417.26666624
TTACGTA650.001580693117.0769234
TTATACG1005.8837995E-616.65000235
TCGCTAT4050.015.9876551
GTGTTAG4650.015.516131
GTATTAA1557.221388E-915.5161291
CCTAGTA2350.014.9574462
CGCTATA3250.014.82
TACGTAG1001.0942461E-414.814
TCCTACA5050.014.6534662
TAATACT2400.014.6458344
GTCTTAG2301.8189894E-1214.4782611
CCTATAC2059.276846E-1114.4390243
Produced by FastQC (version 0.11.5)