FastQCFastQC Report
Thu 28 Apr 2016
ERR1041861.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041861.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6440953
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT76480.11874019263919484No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT73630.11431538159027087No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39600.029.1047951
GTATCAA58600.019.573382
TATACCG2550.016.6862745
GTATTAG8500.016.5411781
TTAACGG3350.016.01492535
GTCGCTA2850.015.57894837
CGCTATA7000.014.82
CTAGTAC2750.014.7999993
GTGTTAG12350.014.6801621
TACGCTG5100.014.5098045
TAAGACT5500.014.4636354
TTTAACG4050.014.16049434
TTAGGAC5000.014.0599993
TATACTG6700.013.805975
GTGTAAG8500.013.7117651
GTATTAA5150.013.6504861
TATCAAC86300.013.4409053
GAGTACG118850.013.38662051
ATCAACG85800.013.3467364
TCAACGC85200.013.332165
Produced by FastQC (version 0.11.5)