FastQCFastQC Report
Thu 28 Apr 2016
ERR1041860.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041860.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5722523
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT113630.1985662617695027No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT107790.18836097294847046No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT76210.133175524152546No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50800.030.6633851
GTATCAA75200.020.6648922
CTAGTAC2750.016.8181823
CGCTATA8300.014.4879522
TAGACAG8450.014.4497055
CGTTATA908.281107E-414.3888892
TATCAAC108400.014.3357933
ATCAACG109050.014.2673094
TCAACGC110050.014.2217175
TATACTG7200.014.1319455
CAACGCA111000.014.0166656
TCGCTAT9250.014.01
TTAGTAC2650.013.9622643
AACGCAG114450.013.6911317
CCTATAC5950.013.6806723
TAATACG2304.0017767E-1113.6739134
GTACTAG2850.013.6315781
GTATAGA5700.013.6315781
TATAGTG5650.013.4247795
TTATACG1951.0284566E-813.2820535
Produced by FastQC (version 0.11.5)