FastQCFastQC Report
Thu 28 Apr 2016
ERR1041859.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041859.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6767501
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT113830.16820093561862792No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT108620.16050237746547805No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT74590.11021793716764873No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55300.030.10851
GTATCAA81200.020.3454442
CGCTATA8050.015.627332
GTATTAG5700.015.5789481
TTATACG1902.7284841E-1115.57894835
GTGTTAG13450.015.1301121
TCGCTAT9150.014.355191
TAGACAG9550.014.3350785
TATCAAC117400.014.2768313
GTTAGCC14350.014.1811853
ATCAACG117600.014.1109694
TCAACGC117950.014.0377285
CTAGTAC2650.013.9622633
TCTATAC3850.013.9350643
GTATTAT3600.013.8751
CAACGCA120350.013.757796
TAGACCG1907.1449904E-913.6315795
TACACTG11650.013.3390555
TATATTG11100.013.3333335
CTACACT8750.013.3200014
Produced by FastQC (version 0.11.5)