FastQCFastQC Report
Thu 28 Apr 2016
ERR1041858.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041858.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4846038
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT81290.16774527975224296No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT75380.15554975012577285No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT53490.11037882905581838No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37950.030.4189721
GTATCAA58100.019.8373492
CGCTATA4750.017.5263162
GTCTTAG6700.016.5671631
GTATAGA4850.016.0206181
GTATTAG6700.015.1865671
TATACTG5200.014.9423075
TAAGACA3750.014.8000014
TCTGTCG11750.014.88
TAGACCG1252.9633175E-614.7999995
TAACCGG3150.014.6825422
GTCTAGA5000.014.4299991
AACGGTA908.280788E-414.38888818
CTAGACT4900.014.3469384
TCGCTAT6200.014.322581
CTAGTAC2201.8189894E-1114.2954543
TATAGAG2900.014.0344832
CCTATAC4650.013.9247313
TAATACT6200.013.7258064
TATATTG6750.013.7037035
Produced by FastQC (version 0.11.5)