FastQCFastQC Report
Thu 28 Apr 2016
ERR1041857.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041857.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5755041
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT98590.1713106822349311No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT91700.15933856943851488No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT64990.11292708427272716No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40100.029.6184521
GTATCAA59400.019.7769362
TACGCTA752.0690812E-417.2666665
TATACCG2800.013.8755
GTATTAG7100.013.8098591
CTAGACA5650.013.7522134
GTTATAG3250.013.6615371
TATACTG7200.013.3611115
GTGTTAG10000.013.3200011
GTACAAG12050.013.203321
GTATTAA4100.013.0853661
ATCAACG90850.013.05283452
TATAGAC3700.013.03
TCAACGC92000.012.9902173
TATCAAC91700.012.9721933
CAACGCA91800.012.8976034
GTCTTAG7500.012.8266661
GTGTATG11150.012.6098651
CGCTATA4550.012.6043952
AGTACTG11600.012.5991375
Produced by FastQC (version 0.11.5)