FastQCFastQC Report
Thu 28 Apr 2016
ERR1041855.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041855.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences250163
Sequences flagged as poor quality0
Sequence length43
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGT3920.1566978330128756No Hit
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT2790.11152728421069463TruSeq Adapter, Index 11 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC750.034.5333331
TATACTG303.5915023E-430.8333345
TATACAC900.030.8333323
TTATACA850.030.4705892
GGTATCA1950.030.3589741
CTAGTCT250.00548822929.5999984
TTGACTA250.00548822929.59999818
ATACACA1250.026.6400014
TAGCACA509.054018E-625.8999984
TACACAT1200.024.6666685
TTGTCAC400.001926989523.12534
CTAGGCA400.001926989523.1254
TACACAC502.6939219E-422.1999995
CTAGCAC502.6939219E-422.1999993
ATCTCCG1201.0913936E-1121.58333410
GTATCAA2800.021.1428572
GATTTGC450.003817570620.5555558
TTACACA450.003817570620.5555554
GCTTTAT450.003817570620.5555551
GTTCTGA555.127649E-420.1818181
Produced by FastQC (version 0.11.5)