FastQCFastQC Report
Thu 28 Apr 2016
ERR1041854.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041854.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1186112
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT35240.29710516376193813No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT33370.28133936761452544No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT21370.18016848324610155No Hit
CCCACGCCCTCTGCATCTTTTTTCTTTTCCCCCACGGATCTTT13180.11111935466465224No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16900.029.0088771
GTATCAA23300.020.8819732
TATACTG1251.8189894E-1120.725
GTATACG450.003825879420.5555551
GTATTGG1101.7553248E-920.1818181
CTTAATA701.21923855E-418.52
TTATACC701.21923855E-418.54
TTAATAC752.0673365E-417.2666663
TAATACT1005.878108E-616.6500024
CCTTATA803.3826803E-416.18752
TGTCCGT803.3826803E-416.187510
GTACTGT1951.8189894E-1216.1282066
TACTAGG1151.2422006E-616.0869562
TAATATA700.002592558315.8571434
TAGCACC1403.4728146E-815.8571434
GTTACAC700.002592558315.8571433
TTAGGCA1059.343525E-615.8571424
TAAGACT1059.343525E-615.8571424
CCTACAC2350.015.7446793
GTGTACA2950.015.6779651
Produced by FastQC (version 0.11.5)