FastQCFastQC Report
Thu 28 Apr 2016
ERR1041853.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041853.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1008827
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT19600.1942850458998421No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT18970.18804016942449003No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT13090.1297546556545374No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10100.032.787131
TAACCGG250.00549602929.622
TAGTATC250.00549602929.64
TTATACG454.0076193E-628.77777735
CGTATGG358.8686397E-426.4285722
TATATAC502.7017848E-422.23
TCTAAGC753.739824E-722.23
GTATCAA15200.021.7861842
CTATACA705.100901E-621.1428594
GTTTAGG1059.822543E-1021.1428571
TTTAGCG450.00382548920.55555526
GCTATAC500.007034318.53
CGCACTA500.007034318.512
TATACTT500.007034318.55
TATACGA701.2190096E-418.536
ATACGAA852.7225808E-517.41176437
TCTATAC752.0669508E-417.2666663
TTAATAT650.001579753517.0769213
CTATACT1107.8052653E-716.8181824
CTACTAG1005.876356E-616.6500021
Produced by FastQC (version 0.11.5)