FastQCFastQC Report
Thu 28 Apr 2016
ERR1041852.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041852.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7730265
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT173550.22450718054296973No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT164000.2121531409337196No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT110710.14321630629739085No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA84900.031.1166081
GTATCAA127450.020.6845822
TATACTG8250.016.1454545
GTGTTAG15450.015.9255681
CGCTATA8800.015.3465892
GTTAGCC15300.014.3888883
TATCAAC185800.014.2284713
ATCAACG185300.014.1770114
TCAACGC185950.014.0876575
TCTATAC4750.014.0210523
TCGCTAT9900.014.0151521
CTAGTAC5150.014.0097083
CAACGCA188000.013.9438836
GTACTAG4000.013.8750011
CTATACT6900.013.6739134
TCTAGAC6500.013.6615393
CTACACT10900.013.5779824
TTACACT7950.013.4968574
AACGCAG195100.013.46489057
TGTTAGC17350.013.2219032
Produced by FastQC (version 0.11.5)