FastQCFastQC Report
Thu 28 Apr 2016
ERR1041851.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041851.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2228225
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT36540.16398703003511764No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT34380.15429321545176095No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT23450.10524071850912722No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19600.030.9591831
GTATCAA29250.020.8717962
TAGACTA1153.0540832E-919.3043485
GTTCTAA2150.018.9302331
TTTAGCG902.1540618E-618.526
TTAGACT1156.412847E-817.6956524
GTATTAG3200.016.7656251
CTAGACT1708.54925E-1116.323534
ATAACCG803.384349E-416.18755
CTACACT2550.015.9607844
GTCGCTA957.0644295E-515.57894837
CGGATAA1902.7284841E-1115.57894825
TAAGATA1557.219569E-915.5161294
TACACTG2800.015.1964295
ATTACTC2201.8189894E-1215.1363643
CCAATAC3800.015.0921063
CCTAGTA2109.094947E-1214.976192
GTCTTAC2109.094947E-1214.976191
ATCAACG41300.014.8268774
CAACGCA40850.014.8090586
Produced by FastQC (version 0.11.5)