FastQCFastQC Report
Thu 28 Apr 2016
ERR1041850.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041850.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3232758
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT98620.305064591905735No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT89750.27762671997099686No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT60820.18813656945555465No Hit
CCCACGCCCTCTGCATCTTTTTTCTTTTCCCCCACGGATCTTT46660.14433496104564586No Hit
GTGCAGAGGGCGTGGGGGAAAAGAAAAAAGATCCGTGGGGGAA34220.10585388699061297No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47450.031.3856681
GTATCAA71950.020.7241132
CTAGTAC1900.018.53
TCTATAC2000.018.53
TATACTG4250.017.847065
CTATACT2950.016.9322034
ACTATAC1452.9849616E-916.5862063
CGCTATA4450.016.2134822
GTACTAG1606.311893E-1016.18751
CTATACG1557.223207E-915.5161294
TATGTCG1201.938046E-615.4166665
GTATTAG3400.015.2352931
ACGCCCT19800.015.2297984
CTACACT4050.015.0740744
CCCACGC20750.014.81
GGTATAT2006.184564E-1114.81
GCGTATA1001.0943379E-414.81
TCGCTAT5050.014.6534641
TAGGACA3950.014.5189874
TTATACT2550.014.5098044
Produced by FastQC (version 0.11.5)