FastQCFastQC Report
Thu 28 Apr 2016
ERR1041849.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041849.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4656847
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT81360.17471048544218867No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT78580.1687407810477776No Hit
CCCTCAGAGAGGCGAGGGTTCGAGGGCACGAGTTCGAGGCCAA63590.1365516195829496No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT56500.12132672600151992No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41950.031.9725861
GTATCAA64450.020.6097762
CGCTATA6450.018.0697672
CTAGTAC1705.456968E-1217.4117643
TCGCTAT7350.016.3605441
ACCTATA2650.016.0566042
GTATTAA2700.015.0740731
GTTAGAC2006.184564E-1114.7999993
GTATACG1001.0945025E-414.7999991
TAAGACT2550.014.5098044
TAATACG1304.4506905E-614.2307694
TATCAAC93950.014.1974463
GTTAGCC10300.014.189323
TAGGACT6800.014.1470594
CGTATAC1051.6573441E-414.0952383
ATCAACG94850.014.0822364
TATACTG4100.013.9878045
TCAACGC96450.013.8678085
CAACGCA98000.013.723986
GTATAGG3950.013.5822781
Produced by FastQC (version 0.11.5)