FastQCFastQC Report
Thu 28 Apr 2016
ERR1041848.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041848.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7733338
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT134900.17443954990716817No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT129340.16724989907333676No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT87030.1125387251921486No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60500.030.3338851
GTATCAA89900.020.2285862
CGCTATA9400.015.7446792
CTATACT8350.015.0658684
GTCGCTA3950.014.98734237
TAGACTG7800.014.7051295
TATACTG9950.014.6884425
TCTATAC6300.014.6825393
GTATTAG8600.014.412791
TCGCTAT10600.014.3113211
TATCAAC131750.013.9013283
ATCAACG130400.013.8891874
TATACCG3600.013.8755
TCAACGC129800.013.8678735
CAACGCA131450.013.69386
GTATACG2457.2759576E-1213.5918361
GTAGGAC11300.013.5884943
TAGACAG11050.013.5610855
GTACTAG4050.013.2469141
AACGCAG137100.013.2374187
Produced by FastQC (version 0.11.5)