FastQCFastQC Report
Thu 28 Apr 2016
ERR1041847.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041847.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4347280
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT51800.11915496586371248No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT50480.11611858449421246No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25200.028.5575391
GTATCAA37850.019.1109642
TCTATAC2600.017.0769233
CTAGTAC2500.017.023
GTATATA3100.016.7096791
GTATACG803.3852734E-416.18751
GTATTAG5350.015.2149521
TATACCG1954.1836756E-1115.1794875
GTACTAG1904.5656634E-1014.6052641
TACGTCG1803.3378456E-914.3888885
CCTACAC7150.013.9720273
TACATAC4300.013.7674423
CTACGCT1907.1431714E-913.6315794
GTGTTAG7500.013.5666661
TTATACG2051.4279067E-913.53658535
GTCTTAG4650.013.5268831
CGCTATA4800.013.4895832
TCGCTAT5900.013.483051
GTATAGG3850.013.4545471
CGCGTAT1102.4588258E-413.45454512
Produced by FastQC (version 0.11.5)