FastQCFastQC Report
Thu 28 Apr 2016
ERR1041846.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041846.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6290950
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACAACCACGACCAATGATATGAAAAACCATCGTTGTATT70620.1122564954418649No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64950.030.1354891
TCGTTGT14350.021.40069834
CATCGTT14700.021.39455832
TCGCTAA7000.021.14285914
CGACCAA17300.019.99710812
ACGACCA17650.019.60056711
CCACGAC18400.019.5054369
GTATCAA101200.019.4323122
TTAACGC2650.018.84905635
CGTCTAA8100.018.043211
TACAACC21750.017.6068974
CGTTGTA17150.017.47521835
GTTGTAT18250.017.33424636
ACCACGA21350.017.0702578
AACCACG21400.016.5981317
ACTACAA22400.016.4352662
TTAGCGA7350.016.36054427
ATCTTAC12950.016.2857151
AACCTCG9600.016.187519
CTCGCTA9700.016.02061813
Produced by FastQC (version 0.11.5)