FastQCFastQC Report
Thu 28 Apr 2016
ERR1041843.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041843.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45243981
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT897040.19826725680925383No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT789410.174478457145493No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT653900.144527511847377No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA455200.021.3570981
CGCTATA61200.016.4444452
GTATTAG128500.016.326071
TAATACT122650.015.03832154
TTAACGG65800.014.98556335
TCGCTAT69700.014.96987151
CTAATAC123800.014.5997573
TATTAGA119700.014.5898092
CTTATAC244200.014.54545637
TAACGGC68900.014.284471536
GTATCAA681900.014.2080222
TCTTATA363450.014.14541237
ATTAGAG108000.013.7037053
TATACTG68900.013.693765
TTTAACG83400.013.46462834
TCTAATA114300.013.239722
CTCTAAT101500.013.08669951
TTAGAGG158150.012.8909274
TACCCCG61300.012.8866235
CGAACTA56050.012.8394329
Produced by FastQC (version 0.11.5)