FastQCFastQC Report
Thu 28 Apr 2016
ERR1041842.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041842.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences186227
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCGCT200.001837978137.021
TGCACTA303.5880052E-430.83333412
GTGATAC250.005484668529.5999983
CATAGCG250.005484668529.5999989
CGCTATA358.841394E-426.428572
ATTAGAG601.3273766E-624.6666683
GTATTAG802.6948328E-823.1251
GTTTAGG400.001925148423.1251
ACTGTGC502.6903363E-422.1999998
GTATCAA1005.2568794E-1022.1999991
TTAGAGG855.1220923E-821.7647064
GGCACCG806.89588E-720.81259
TATTAGA806.89588E-720.81252
CCCACGC450.003813958520.5555551
TCGCTAT450.003813958520.5555551
GTGTACA450.003813958520.5555551
CAAGACG450.003813958520.55555528
GAGGCAC1001.2723831E-820.3499987
TCTCTAG555.120882E-420.1818181
GCTATAT656.86251E-519.9230773
Produced by FastQC (version 0.11.5)