FastQCFastQC Report
Thu 28 Apr 2016
ERR1041841.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041841.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31464484
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT444620.14130853059595702No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT399070.1268318908392078No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT321680.10223590509223032No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA236450.020.8824271
TTAACGG54000.017.88333135
TAACGGC55100.017.42558936
GTATTAG109550.016.9885881
TTTAACG65100.015.99923134
TATTAGA93700.015.9530412
ATTAGAG84800.015.4239383
CGCTATA25450.014.8290762
CGTGCGA13050.014.60153210
TAATACT100000.014.59649854
AACGGCC67000.014.49626837
CTAATAC94250.014.4270543
TATACTG49750.014.3165825
GTATCAA349600.014.0390432
TCTATAC36750.013.9442173
TCGCTAA17150.013.69970814
TCTTATA191550.013.6854637
CGACCAA38400.013.68229112
CGAACTA52350.013.67621729
TTAGAGG118400.013.656254
Produced by FastQC (version 0.11.5)