FastQCFastQC Report
Thu 28 Apr 2016
ERR1041840.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041840.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27309507
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT295760.10829928200461474No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA224750.022.9984421
GTATTAG104700.017.1041071
TATTAGA90050.016.5380342
ATTAGAG80800.015.7982673
TTAACGG61950.015.55851435
GTATCAA330700.015.5574542
TCGCTAA15850.015.406939514
TAACGGC63100.015.24564236
TTTAACG69600.015.15086134
CGCTATA30700.015.0048862
TTAGAGG115500.014.2393934
TAATACT85650.014.1476944
TCTATAC23900.014.0878673
CGAACTA54250.013.98156729
TCTTATA206050.013.76389337
TTTAGCG18400.013.67391326
TATACTG35050.013.6176895
CTTATAC146650.013.57381537
TACCCCG43350.013.4855825
ATCGATA18300.013.34426323
Produced by FastQC (version 0.11.5)