FastQCFastQC Report
Thu 28 Apr 2016
ERR1041836.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041836.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6056824
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT73580.12148281013283528No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT67430.11132897373276819No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA85800.030.8548971
TTAACGC2350.020.46808635
GTATCAA138150.019.1226922
ATACCGA2250.018.0888886
GTATTAG20950.017.6610971
TATACTG5150.017.601945
ATTAGAG17400.016.9051723
CGCTATA8450.016.6390532
TATTAGA19500.016.1282062
TATATAC14550.015.7663234
CTAGTAC5550.015.6666673
TCGCTAA3900.015.65384714
TATATCG1557.226845E-915.51612955
CTTATAC49300.015.4229237
TTAGGAC10900.015.2752293
ATACCGC14300.015.2657347
TATACAC18250.015.20547937
TAGTACT7250.015.0551724
GTACTAT9750.014.9897451
GTACTAG4450.014.9662911
Produced by FastQC (version 0.11.5)