FastQCFastQC Report
Thu 28 Apr 2016
ERR1041834.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041834.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3124068
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18950.025.4802111
TCGCTAA2200.022.70454614
TATTAGA4600.018.4999982
TTCTATA2350.018.1063842
CGCCTTA3000.017.26666624
GTATCAA28000.016.9803562
ATTAGAG4400.016.8181823
GTATTAG5600.016.5178571
TATATCG700.002593830715.8571425
ATTAGAC1802.0190782E-1015.4166673
CGCTATA4450.015.3820222
TATAGAC1954.1836756E-1115.1794873
CGTTCGT1101.4533782E-515.13636424
CGTATTA1353.9782026E-715.07407515
TAGACTA1601.0984877E-815.0312515
GCTTACC4500.014.828
TATAAGA2500.014.7999992
TCTTATA23900.014.55230137
GTCTAGT2059.276846E-1114.4390241
TTAGAGG7450.014.4026844
Produced by FastQC (version 0.11.5)