FastQCFastQC Report
Thu 28 Apr 2016
ERR1041831.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041831.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5447703
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33800.025.6153831
TCGCTAA1950.018.9743614
TATTAGA13400.018.776122
ATTAGAC3700.018.5000023
GTATCAA49500.017.4161622
TTAACGC1502.5283953E-1017.26666635
GTATTAG16100.016.8913041
GTACTAG3050.015.7704931
TATACCG5400.015.7592596
ATTAGAG14150.014.9045933
TATAACT7700.014.6558444
TAATAGT5000.014.4299994
CGCTATA5000.014.4299992
ATACCGC6100.014.2540997
TTTAACG11400.014.11842134
CGTATTA3200.013.87500115
TCTTATA45350.013.86990137
TTAGAGG19000.013.7289484
CTTATAC31300.013.712460537
CCTATAT7850.013.668792
Produced by FastQC (version 0.11.5)