FastQCFastQC Report
Thu 28 Apr 2016
ERR1041829.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041829.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8888453
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA70850.025.7981641
GTATTAG19700.018.0304581
GTATCAA102050.017.89272
TCGCTAA2750.017.49090814
TATTAGA17050.017.3607042
TTAACGC2250.015.62222235
TACCCCG13000.015.51153855
ATTAGAG17450.014.8424073
ATCTTAC14550.014.7491411
TTAGTAC4200.014.5357143
CTTATAC66600.014.537
TATACTG9950.014.3165835
TCTTATA100600.014.30715737
CGCTATA8700.014.2471272
TAATACT16600.014.1536154
TATACAC28450.014.04569337
TTAGACT6850.014.0437964
ATCTAGT8350.013.9580841
CGAACTA7300.013.93835629
GTTTACC12450.013.22489815
Produced by FastQC (version 0.11.5)