FastQCFastQC Report
Thu 28 Apr 2016
ERR1041828.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041828.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2039656
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG25580.12541330498868436No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC24860.12188329796789263No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA23280.1141368936722663No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGC1150.033.78260835
GGTATCA20650.029.4745771
TAACGCT1400.026.42857236
AACGCTG2100.022.0238137
GTATCAA30850.019.7293342
GAGCGTA609.240162E-418.56
TCTAACG609.240162E-418.52
AACCTCG4200.018.519
GCGGAAT2300.018.519
TATACTG1451.546141E-1017.8620685
GCCTTAC4400.017.6590925
ATCGTAC1054.801768E-717.61904725
ACCTCGC4550.017.48351720
TCGCTAA4600.017.29347814
CGTATCT650.001580501217.0769239
GTACGGA2600.017.0769236
GTATACG1851.8189894E-1217.01
CTCGCTA5050.016.85148613
GTATAAA2000.016.6500021
GTATTAG12000.016.651
Produced by FastQC (version 0.11.5)