FastQCFastQC Report
Thu 28 Apr 2016
ERR1041827.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041827.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10011471
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT145060.1448937923308173No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT138080.13792178991478876No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA115000.029.1013051
TTAACGC2850.018.17543835
GTATCAA183450.018.1722552
TATACTG10300.018.1407785
TATTAGA32950.017.910472
ATTAGAG29650.017.8448563
GTATTAG38250.017.5084971
TCGCTAA9000.016.03333314
TTAGAGG43350.015.7474044
TTTAACG27850.015.34470434
TAATACT34000.015.2897064
TTAACGG24300.015.226336535
CGCTATA14500.014.5448272
TAACGGC25450.014.5383136
CTAATAC35150.014.5263163
AATACTG32000.014.5109375
GTACTAG5750.014.4782611
GGCACCG33800.014.0665679
CTCTAAT29150.014.0257291
TTAGGAC16200.013.9320983
Produced by FastQC (version 0.11.5)