FastQCFastQC Report
Thu 28 Apr 2016
ERR1041825.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041825.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12303790
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA109700.026.0214211
TTAACGC3400.021.22058935
GTATCAA155600.018.1908742
CGCTATA11050.017.0769212
TATTAGA22500.016.9377772
GTATTAG27150.016.8305721
CTTATAC83250.014.977777537
ATTAGAC6350.014.8582683
TAATACT21700.014.8341024
TATACTG14750.014.85
TCGCTAT12900.014.7713181
TCGCTAA5450.014.59633214
TACCCCG13700.014.5839415
ATTAGAG22800.014.5241223
TATACAC32300.014.37616237
GTATTAA8700.013.8218381
TCTTATA122250.013.58936737
TTAATAC9500.013.4368423
CTAATAC23500.013.30425553
GTCCTAA16900.013.2455631
Produced by FastQC (version 0.11.5)