FastQCFastQC Report
Thu 28 Apr 2016
ERR1041821.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041821.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences686919
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG509.084135E-625.9000025
ACATATA509.084135E-625.9000021
GGTATCA5650.025.5398241
TAGCGGA502.7005686E-422.217
GTAAGGC1151.3460522E-1020.91304216
TTTCGTG555.1401986E-420.18181824
TTGGTCG555.1401986E-420.18181810
AGTTATA555.1401986E-420.18181835
CTATTAA555.1401986E-420.1818181
CATATAA656.896963E-519.9230772
CTAATGG656.896963E-519.9230773
TCAGTTA759.256872E-619.73333433
TATGAGT851.2438159E-619.5882344
TTAGCGA951.6736703E-719.47368227
TAATAGT1153.0431693E-919.3043464
GTGTAGG1153.0431693E-919.3043461
TACACTG1254.110916E-1019.245
AATAGTG1355.638867E-1119.1851845
GTATTGA801.6151276E-518.51
CTAAGCC500.00703207118.53
Produced by FastQC (version 0.11.5)