FastQCFastQC Report
Thu 28 Apr 2016
ERR1041820.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041820.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15711923
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT278030.17695478777486373No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT258560.16456292460190902No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC231390.14727032458089312No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG214010.136208661409555No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAT212350.13515213892023273No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT181990.1158292336335915No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG177930.11324520875006835No Hit
TCCCAATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTCAACGA170720.10865633697415651No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC167840.10682333410111544No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGAC158790.10106337715631625No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC158470.1008597101704228No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA249250.027.7741241
TTAACGC8100.023.06790235
TATTAGA79100.018.9677622
GTATTAG93450.018.9058321
TCGCTAA19600.018.78316514
GTATCAA370850.018.6770942
CTCGCTA20150.018.45409413
ATTAGAG76550.018.197913
TTAGAGG96400.017.4636944
GGCACCG79200.016.8882589
ATCTTAC42200.016.8779621
TACCCCG42350.016.7308165
TATACAC38700.016.06201637
GGTAAAC52650.015.81196635
AATACTG65100.015.7434715
TAATACT74000.015.7254
GCCTTAC24150.015.70393425
GTTTACC43500.015.69310415
ACCGCCT85100.015.60869612
CAGTATT44400.015.45833314
Produced by FastQC (version 0.11.5)