FastQCFastQC Report
Thu 28 Apr 2016
ERR1041819.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041819.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7314317
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAT174450.23850483920781668No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC151360.20693661486096376No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT147780.20204210454646687No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG139020.1900655932741225No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT139010.19005192145760158No Hit
TCCCAATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTCAACGA127590.1744387069906869No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA121740.1664406943259364No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGAC108670.14857163013306643No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC104070.14228259453343353No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC103620.1416673627899912No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG102680.14038221203702272No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT102060.13953455941272438No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA100110.13686855519114088No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG93680.12807757716817578No Hit
ATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCA89940.12296431778934383No Hit
TATCAACGCAGAGTACGGGGAGCGGATAACAATTTCACACATA85820.11733152938271613No Hit
GGTATCAACGCAGAGTACGGGGAGCGGATAACAATTTCACACA80620.11022218479182677No Hit
ACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCAA80590.11018116934226395No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG79180.10825344321281126No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA78050.10670852794594493No Hit
GTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGAT77840.10642141979900516No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT76740.10491751998170165No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG75730.1035366665130866No Hit
TTCCTTATGAGCATGCCTGTGTTGGGTTGACAGTGAGGGTAAT74200.1014448785853826No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGC4850.029.75257735
GGTATCA173050.029.0889911
TCGTTGT5600.022.46428734
TGCGACG3350.021.53731222
CATCGTT5950.021.45378132
ATCTTAC22250.020.287641
TATACAC20800.020.10096237
TCGCTAA12600.019.96825414
GTCTTAC4150.019.6144581
TATTAGA51100.019.5136992
GTATTAG59850.019.4736861
ACCTCGC13350.019.2621720
GTATCAA263950.019.0712262
CAATGCG3800.018.98684319
ATTAGAG49350.018.9685923
GTACTAG4400.018.9204541
TTACCAA23250.018.61935617
CGACGAG4200.018.524
CAGTATT23900.018.42259414
GCCTTAC13900.018.10072125
Produced by FastQC (version 0.11.5)