FastQCFastQC Report
Thu 28 Apr 2016
ERR1041818.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041818.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51457435
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1659950.3225870080776471No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1487410.2890563822312558No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1191790.23160695825588662No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG730910.14204166997441672No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC717200.13937733196378715No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG663600.1289609557880217No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAT575830.11190413980020573No Hit
TCCCAATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTCAACGA534420.10385671186292125No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC517460.10056078387894772No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1179400.025.1555461
TTAACGC20050.022.88279235
TATTAGA244600.017.9554372
GTATTAG278100.017.9212511
ATTAGAG231650.017.441833
TATACAC134350.017.36397637
TCGCTAA55750.017.25560614
CGCTATA85550.016.8889542
GGTAAAC163100.016.68516335
TAATACT238350.016.610034
GTATCAA1793500.016.52882
AATACTG189750.016.5159425
ATCTTAC132750.016.319021
TTAGAGG311850.016.1656254
TACCCCG137100.016.0171415
GTTTACC132250.015.98903615
GGCACCG242150.015.8374989
TCGCTAT95700.015.7742941
TTGGTAA174000.015.74626433
GTGACAC244600.015.5805424
Produced by FastQC (version 0.11.5)