FastQCFastQC Report
Thu 28 Apr 2016
ERR1041817.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041817.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1769548
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT42460.2399482805778651No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT39780.22480317007507003No Hit
TATCAACGCAGAGTACGGGGAGCGGATAACAATTTCACACATA37570.21231410507089946No Hit
GGTATCAACGCAGAGTACGGGGAGCGGATAACAATTTCACACA32940.18614923132913036No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT27480.15529389426000312No Hit
GTATCAACGCAGAGTACGGGGAGCGGATAACAATTTCACACAT25810.14585645599893307No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC24710.13964017930002465No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAT24340.13754924986493725No Hit
TCTCTAGTATGTGTGAAATTGTTATCCGCTCCCCGTACTCTGC21530.12166948847954394No Hit
CCCACGCCCTCTGCATCTTTTTTCTTTTCCCCCACGGATCTTT18980.10725902885934714No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGT17840.10081670573502387No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49600.030.286291
TTAACGC856.91216E-1126.11764535
GTACTAG1200.023.1249981
TACGCAT1800.020.55555528
CTTACGC450.003826612120.5555553
GTATCAA75300.019.9249672
GCCTTAC2600.019.92307725
TACACGC759.270405E-619.7333345
TCGCTAA2650.019.54716914
TTGGTAA4150.019.16867433
ATTAGAG8600.018.7151173
ACTATTA2600.018.537
GGTAAAC4300.018.535
ACCGCGT500.00703634518.58
CGACCGG1800.018.531
AATGCGA1205.178663E-918.520
TGCGACG1205.178663E-918.522
GTATTAG9850.018.406091
ACCTCGC3050.018.19672220
TAAACAG4700.018.10638237
Produced by FastQC (version 0.11.5)