FastQCFastQC Report
Thu 28 Apr 2016
ERR1041816.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041816.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68408974
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT6844191.0004813111215496No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6311340.922589483654586No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT4572710.668437155628149No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1200400.17547405403273553No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2170000.026.9972131
GTATCAA3250900.018.0202712
CGCTATA155000.017.151292
TCGCTAT157700.016.9632231
CTTATAC608750.015.75121237
TATACAC261600.015.60760837
GTATTAG123300.014.5239251
GCTTACC92200.014.48698528
TATACTG94850.014.33579355
ATCTAGT152600.013.8446911
GCTATAT206350.013.6273333
ACGCCCT394250.013.5142684
TCTTATA947300.013.48099837
TAATACT126900.013.0768324
TCTATAC58450.013.07185653
GTACTAG43250.013.0034691
CCCACGC411550.012.955171
TATATTG249800.012.8714975
TTAACGG50150.012.80059935
TAGACCG12250.012.6857155
Produced by FastQC (version 0.11.5)