FastQCFastQC Report
Thu 28 Apr 2016
ERR1041815.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041815.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41655943
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1013170.24322339791947573No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT971080.23311919742160203No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC807780.19391710805826676No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT671300.16115347574774624No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG642470.1542324945086467No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG620570.14897514143420063No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAT592310.14219099541210722No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC541420.1299742512130862No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC528840.1269542739675825No Hit
TCCCAATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTCAACGA524900.12600843053775063No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA518980.12458726477516066No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA490020.11763507550411234No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGAC471220.11312191396075225No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG446750.1072476021008575No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT437420.10500782565407293No Hit
ATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCA435470.10453970517484143No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG429680.1031497474442002No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG420280.10089316667252017No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA765250.030.3518451
TTAACGC16600.024.8524135
GTATCAA1193450.019.4634062
TATTAGA245750.018.9102762
ATTAGAG229150.018.3748633
GTATTAG286200.018.209121
CGTAGCG34300.017.25947615
TTAGAGG298550.017.1584344
GGCACCG235050.017.1265689
TTTAGCG36450.017.05349726
GTGACAC238050.016.9962224
GACACAT237850.016.99495526
CGCTATA68450.016.8918912
TCGCTAA60050.016.57452214
ACCGCCT244800.016.55024512
CACATGT244300.016.4705328
ACATGTT243250.016.38951529
ATCTTAC121150.016.2781681
GCGGAAT36900.016.24390219
CAGTATT122750.016.18655814
Produced by FastQC (version 0.11.5)