FastQCFastQC Report
Thu 28 Apr 2016
ERR1041814.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041814.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15063163
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT175540.11653594932219746No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT171510.11386054841204334No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA262800.028.700341
GTATCAA379500.019.8210812
GCTTACC29450.018.78268228
GGGCTTA31050.017.99355926
TATACTG14800.016.255
TATACAC55450.016.18124437
CTTACCT33550.016.15648329
CGCTATA28950.015.8480142
TCGCTAT30250.015.4727281
CTTATAC129200.014.533668537
GTACTAG8950.014.4692731
GTATTAG19250.014.4155851
GGCTTAC38400.014.40494827
TAATACT17100.014.2807024
TATATTG39100.014.0524295
ATCTAGT16750.013.805971
TTAATAC12500.013.7640013
GGTAAGC33350.013.479768
AGGTAAG33100.013.41389757
GTGTACA46100.013.4034711
Produced by FastQC (version 0.11.5)