FastQCFastQC Report
Thu 28 Apr 2016
ERR1041811.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041811.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8678855
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAT189890.21879614303960604No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC188450.2171369379947009No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG157060.18096857246722062No Hit
TCCCAATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTCAACGA133470.15378756759964304No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA127110.14645941198464543No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT125720.14485781822602176No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG125040.14407430473259433No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC121920.14047936046863327No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT120890.13929256797123585No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGAC120500.1388431999382407No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC120370.13869341059390897No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA119040.137160950378823No Hit
TATCAACGCAGAGTACGGGGAGCGGATAACAATTTCACACATA107080.1233803307003055No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG102150.11769985787295674No Hit
GGTATCAACGCAGAGTACGGGGAGCGGATAACAATTTCACACA101460.11690482212227304No Hit
ATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCA92920.10706481442540519No Hit
GTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGAT90140.10386162690815783No Hit
CATTCATACAGGTCCCTAATTAAGGAACAAGTGATTATGCTAC90000.10370031530656983No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA87900.10128064128274986No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT87540.10086584002152357No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGC5650.027.50442535
GGTATCA145500.027.5020621
TCGCTAA7950.019.31446514
TATTAGA58300.019.0077192
ATTAGAG55350.018.4832883
GTATCAA218500.018.313732
ATCTTAC23000.018.178261
TGCGACG4500.018.08888822
TAACGCT8950.017.9832436
GTATTAG70050.017.9586011
AACGCTG9150.017.59016437
GTACTAG5850.017.3931621
TTAGAGG69050.017.3613324
CAGTATT29950.017.35726214
GGCACCG58950.017.0720969
CTCGCTA9200.016.89130413
ATCGATA4600.016.89130423
CGACGAG5150.016.52427124
GTGACAC61450.016.49796524
CGACCAA10600.016.4056612
Produced by FastQC (version 0.11.5)