FastQCFastQC Report
Thu 28 Apr 2016
ERR1041809.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041809.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3969503
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACGGGGAGCGGATAACAATTTCACACATA40480.10197750196939011No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49150.030.1871831
TTAACGC1350.023.29629735
GTATCAA71700.020.641562
TCGCTAA1800.020.55555514
ACATATA3500.020.0857141
CGTATTA951.678709E-719.47368415
ACGTATA500.007037849618.531
TAACGCT1750.017.9714336
ATATAAC4350.017.8620683
GTATTAG10800.017.6435181
TATAACT4850.017.164954
GTATACG650.001580858117.0769211
TATTAGA9550.017.0471212
CATATAA4450.016.6292152
TATGCCG7000.015.85714333
TATTAGG3150.015.8571422
TTTAGCG1302.5934423E-715.65384526
TTAGCGA1455.3560143E-815.31034527
TATATAG2900.015.3103452
ATTAGAG9550.015.3036663
Produced by FastQC (version 0.11.5)