FastQCFastQC Report
Thu 28 Apr 2016
ERR1041808.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041808.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11995849
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACGGGGAGCGGATAACAATTTCACACATA211830.17658608406958107No Hit
GGTATCAACGCAGAGTACGGGGAGCGGATAACAATTTCACACA202160.16852496225986172No Hit
GTATCAACGCAGAGTACGGGGAGCGGATAACAATTTCACACAT158520.13214571140400316No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT130130.10847919142696778No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT128940.10748718160757109No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA173050.029.3134921
GTATCAA256500.019.6900582
GTATTAG32000.018.03751
TATTAGA26600.016.5526312
TTAACGC2850.016.2280735
ATTAGAG25100.015.9203193
CGCTATA15400.015.2564932
ATCTAGT26550.014.5630881
CTTATAC74250.014.5010137
TACCCCG17550.013.914535
TATACTG15700.013.9044595
TTAGAGG35000.013.9014294
TCTTATA108800.013.89200337
TTACGCG2009.877112E-1013.87500119
TCTATAC9200.013.8753
ATCTTAC18950.013.8627971
TCGCTAT18100.013.4917131
TAATACT28350.013.4426814
ATCAACG379900.013.3186374
CAACGCA380900.013.2690996
Produced by FastQC (version 0.11.5)