FastQCFastQC Report
Thu 28 Apr 2016
ERR1041805.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041805.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136795
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT6890.5036733798749954TruSeq Adapter, Index 11 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGT3260.23831280383054937No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2900.2119960524872985No Hit
CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC2900.2119960524872985No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2850.20834094813406923No Hit
CTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTC1920.14035600716400454RNA PCR Primer, Index 11 (95% over 22bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1810.1323147775869001No Hit
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCC1780.13012171497496253No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATG1560.1140392558207537No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2750.030.2727261
GACTGGT250.005479634729.67
CTTAGGC250.005479634729.63
CACTGTA358.8293315E-426.428572
CGTCTTC451.3149026E-424.66666637
CAGTGCT400.001922546223.1250029
GTATCAA3850.021.6233792
ACTGCTC450.003808854120.5555558
TAGAAGT450.003808854120.5555554
GGCATCA450.003808854120.55555513
TAATGTT450.003808854120.5555555
ACTGGTG450.003808854120.5555558
CTCTTAT1900.020.44736937
CATGCAC555.1113183E-420.18181810
TAATCAC500.00700400518.526
TCTTAGG500.00700400518.52
CCGTCTT1054.7213507E-717.61904737
CAACGCA5000.016.2799996
AACGCAG5000.016.2799997
GCCGTCT1151.2226665E-616.08695636
Produced by FastQC (version 0.11.5)