FastQCFastQC Report
Thu 28 Apr 2016
ERR1041801.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041801.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3713351
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT44780.12059188587343346No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT44660.12026872762634074No Hit
TATCAACGCAGAGTACGGGGAGCGGATAACAATTTCACACATA40030.10780020525934661No Hit
GGTATCAACGCAGAGTACGGGGAGCGGATAACAATTTCACACA38410.10343756892359489No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA79250.030.7671931
GTATCAA116400.020.9158082
GCGGAAT2450.020.38775419
CGCGAAA656.907435E-519.92307515
GCTTACC5200.019.56730728
TCGTAGC2800.018.514
TAGCGGA2800.017.83928517
CGTAGCG2900.017.22413815
CGTCTAA2450.016.6122441
GTCGTAG3250.016.50769213
GTCGTAT1151.243945E-616.08695613
TATACTG3250.015.9384615
GTATAAA3950.015.924051
TATACAC14700.015.73129337
GTATTAA3550.015.6338031
TATAGTG4300.015.4883725
AATCGGG3350.015.46268723
CTTACCT6700.015.46268729
CTAGACT3600.015.4166664
TTAGTAC1601.0988515E-815.031253
Produced by FastQC (version 0.11.5)