FastQCFastQC Report
Thu 28 Apr 2016
ERR1041799.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041799.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6139343
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACGGGGAGCGGATAACAATTTCACACATA86760.141318053088091No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG82810.13488413988272033No Hit
GGTATCAACGCAGAGTACGGGGAGCGGATAACAATTTCACACA81350.13250603525491245No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT74310.12103901020027713No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT74080.12066437727945808No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGC2600.024.90384735
GGTATCA187400.022.0933821
GTATTAG25750.019.3980581
ATTAGAG20250.019.0938283
TATTAGA23300.018.8175962
TCGCTAA7150.017.85314814
CGTATTA4000.017.57515
TTTAGCG4450.017.46067426
ATTAGAC3850.017.29873
TTAGAGG25150.016.918494
GTATCAA252400.016.4110552
GCTTACC7950.016.28930728
TATACTG6150.016.2439025
GGGCTTA8000.016.187526
ATCTTAC12350.015.8785421
TATACAC24400.015.84631237
GTACTAG3750.015.7866681
CGCTATA3650.015.712332
TTAGCGA7800.015.65384727
ACCGCCT24500.015.63061212
Produced by FastQC (version 0.11.5)