FastQCFastQC Report
Thu 28 Apr 2016
ERR1041798.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041798.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6713822
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT375450.55921947290232No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT302070.44992256273699244No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT188680.28103217511575374No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT118760.1768888123635092No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104760.1560363083799362No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG93290.13895214975910888No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG85890.12793011193922033No Hit
TATCAACGCAGAGTACGGGTCATCTCCCCCAGATCTTGACTAT74360.11075658544417769No Hit
TCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTG73150.10895433331416889No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTAGA21000.014.0071442
ACTAGAT12200.013.49590132
GTACTAG11400.013.46929930
ATTAGAG21000.013.3904773
TACCCCG12550.013.26693255
TAATACT20650.013.2590794
GGGGTTA11550.012.9740266
TATACTG5950.012.7478995
ATACCGA2653.274181E-1112.5660386
TTAATAC4000.012.4874993
GGGTTAG11600.012.2801727
CTTACCC13300.012.2406023
GCTATAC4850.012.2061853
TAGACAG8700.012.120695
CGAACTA22950.012.09150324
TTAACGG18700.012.06951835
TCTAATA16150.011.9133132
CCGAGTT7300.011.91095913
GTTTACC12500.011.8415
CGTACTA13050.011.76628429
Produced by FastQC (version 0.11.5)