FastQCFastQC Report
Thu 28 Apr 2016
ERR1041796.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041796.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9539736
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT564510.5917459351076382No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT478920.5020264711727872No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT352930.3699578269251895No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181870.1906446886999808No Hit
GTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT96110.10074702276876425No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA387400.015.4819311
TATTAGA29600.014.81252
ATTAGAG27600.014.545293
TTAACGG24350.014.28336735
GTATTAG40950.013.6434671
TAACGGC25650.013.487329536
GGGGTTA13650.013.2820516
GTATAGA8450.012.6982241
TCTAATA26000.012.5230782
AATACTG27500.012.5127275
TAATACT33150.012.4449474
GTTTAAC29700.012.20875433
TTTAACG29000.012.18448334
ACACCGT7600.012.1710536
ACCGCCT31900.012.00470312
AACGGCC29600.011.812537
TTAGAGG44800.011.8102684
AACCGTA3450.011.7971027
GTATCAA508900.011.7783452
GTACTAT11350.011.73568251
Produced by FastQC (version 0.11.5)