FastQCFastQC Report
Thu 28 Apr 2016
ERR1041795.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041795.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8281681
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT298340.3602408738032774No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT297090.3587315183958426No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT198510.2396977135439049No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG101800.1229219043814897No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100270.1210744533627895No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG90630.10943430446065237No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC86340.10425419670233614No Hit
TCCCAATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTCAACGA85320.10302256268986937No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA173150.014.7230721
TTAACGG24800.013.50201535
TATTAGA30250.013.3933882
TCTATAC6700.013.2537323
ATTAGAG28250.013.2283183
TAACGGC25600.013.08007936
GGGGTTA15000.012.8266686
TACCCCG15600.012.8076935
GGGTAAG17100.012.7660821
GTATTAG41650.012.7034821
CGTATCG4100.012.63414710
CCGAGTT12600.012.33333413
CGAACTA30850.012.23338824
TTAGACT7450.012.1677854
TCTAATA29600.012.02
AATACTG31200.011.9775655
CGCGTAT1705.3191507E-611.97058912
TTTAACG29150.011.9313934
ACCGCCT30100.011.86212712
GCGAACT32250.011.81705523
Produced by FastQC (version 0.11.5)