FastQCFastQC Report
Thu 28 Apr 2016
ERR1041794.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041794.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10531264
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT443080.4207282240764261No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT422150.40085406652041006No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT339440.3223164854665119No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144300.13702058936135303No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC106850.10145980577450152No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA217800.016.9370981
TAATACT38600.015.7202064
TTAACGG25500.015.45294135
TAACGGC26250.014.94095136
CTCTAAT29100.014.8762881
AATACTG35550.014.5710285
TCTAATA33100.014.419942
TATTAGA35350.013.8684582
ATTAGAG33050.013.8260213
GGTAAAC29050.013.62822735
TACCCCG18600.013.4274195
ATGCTAG30700.013.25732915
TTTAACG30900.013.23139334
ATACTGG36650.013.0736696
TACTGGT34850.013.0588247
CTAATAC44300.012.9875853
GTATTAG47300.012.9460891
CTACGCT5500.012.78181830
GATGCTA31850.012.60439514
AACGGCC31300.012.589457537
Produced by FastQC (version 0.11.5)