FastQCFastQC Report
Thu 28 Apr 2016
ERR1041793.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041793.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10667893
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT255060.23909126197647462No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT253070.2372258514403922No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT175500.16451233622234496No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC141650.1327816092643599No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG135190.12672605546381088No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG114920.10772511497818735No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGG35050.016.20399535
TATTAGA43050.015.7282222
ATTAGAG40650.015.7011073
CTCTAAT31550.015.5974651
TAACGGC36450.015.48010936
TCTAATA34350.015.241632
AATACTG38300.014.9738915
TAATACT44000.014.8840914
GTATTAG58050.014.6279071
TTTAACG40850.014.492043534
GGTATCA182750.014.4558141
ATGCTAG32350.014.41112815
GGTAAAC31550.014.24881135
GTTTAAC41850.014.18996433
ATACTGG39300.014.0750656
GGGGTTA16000.013.9906246
TTAGAGG57950.013.88697054
GTGACAC42750.013.84795324
AACGGCC41200.013.83009637
AACTAGA24900.013.81927727
Produced by FastQC (version 0.11.5)