FastQCFastQC Report
Thu 28 Apr 2016
ERR1041792.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041792.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9231917
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT147190.15943600879427317No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT142930.15482158255972187No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC103500.1121110599239573No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG101490.10993383064427464No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT101380.10981467879314774No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA94950.018.5876791
CTCTAAT25800.015.9903111
AATACTG29200.015.8390415
TTAACGG25750.015.59029235
TCTAATA28150.015.5097692
TAATACT33100.015.3700914
TATTAGA32000.014.9734382
ATTAGAG31250.014.6816013
ATACTGG30100.014.5049846
TATACTG8800.014.2954545
ATGCTAG25950.014.2581915
TAACGGC27700.014.22563236
GGTAAAC26100.014.17624535
GTATTAG45400.013.8138761
TACTGGT30400.013.7532897
GATGCTA27100.013.51660414
TAGGACC18100.013.3895034
TTGGTAA27600.013.33876833
CTAATAC42150.013.167263
GGGGTTA14400.013.1041676
Produced by FastQC (version 0.11.5)