FastQCFastQC Report
Thu 28 Apr 2016
ERR1041791.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041791.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5291901
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT89230.16861615513971254No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT84250.15920554825194197No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG63040.11912543337450945No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC53320.10075774282247534No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT53100.10034201320092723No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAGAG15550.014.6334413
TATTAGA16700.014.5119762
TAATACT18550.014.3611864
TTAACGG14700.014.22108835
CTCTAAT14150.013.7279151
AATACTG17400.013.7155185
GTATTAG22850.013.4398241
GGTATCA83450.013.4122231
TAACGGC15450.013.41100436
TCTAATA15800.013.2310122
GGGTTAG9550.013.1727747
TACTCCG24050.012.9230785
ATACTGG17600.012.8238646
CTAATAC23500.012.7531923
TAGACCG1602.7000187E-612.7187515
ATGCTAG14700.012.71088415
CTAGTAC1755.315469E-712.6857143
GGGGTTA9050.012.6740346
CCGAGTT7650.012.57516313
TATACTG4900.012.4591845
Produced by FastQC (version 0.11.5)