FastQCFastQC Report
Thu 28 Apr 2016
ERR1041790.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041790.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5149978
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT340790.6617309821517684No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT325750.632526973901636No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT182220.3538267542113772No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99750.19369014780257315No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC70660.13720446961132649No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG68510.1330296944957823No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG54200.10524316802906732No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA114500.019.889521
CCGAGTT9100.016.6703313
TATTAGA16700.015.0658682
GTATCAA152600.014.8994112
GGGTAAG11850.014.83122351
TGCTCGG21800.014.5963310
TTGCCGA10700.014.52336410
GCTCGGA21850.014.39359311
ATTAGAG14300.014.360143
GCCGAGT10750.014.28372212
GTCGCCC20100.014.2661737
AGGTCGC20350.014.18181835
TGCCGAG11300.013.58849411
TACCCCG9300.013.5268835
ACTGCTC27900.013.3942658
CTACACT7800.013.2820524
CGAACTA17000.013.16764724
GCTATAC4650.013.1290333
GCTCCGA23300.013.10085929
CTGCTCG26000.013.0923089
Produced by FastQC (version 0.11.5)